Dynamic interplay between human alpha-satellite DNA structure …
网页2024年3月15日 · Mitotic chromosomes show repetitive alpha-satellite DNA staining depicting a ring-like structure with some examples of unstained DNA in the center of the ring, presumably from flanking pericentromeric DNA.
Alpha-synuclein is a DNA binding protein that modulates DNA …
网页2019年7月29日 · Alpha-synuclein binds double-stranded DNA and helps to facilitate the non-homologous end-joining reaction. Using a new, in vivo imaging approach that we developed, we find that serine-129-phosphorylated alpha-synuclein is rapidly recruited to DNA damage sites in living mouse cortex.
α satellite DNA variation and function of the human centromere
网页2017年4月13日 · Satellite DNA, including α satellite DNA found at human centromeres, comprises up to 10% of the genome, but is difficult to study because its repetitive nature hinders contiguous sequence assemblies. We recently described variation within α satellite DNA that affects centromere function.
Alpha-synuclein - Wikipedia
网页Alpha-synuclein is a synuclein protein primarily found in neural tissue, making up as much as one percent of all proteins in the cytosol of brain cells. [17] . It is expressed highly in neurons within the frontal cortex, hippocampus, striatum, and olfactory bulb, [17] but can also be found in the non-neuronal glial cells. [18] .
Alpha-synuclein is a DNA binding protein that modulates DNA …
网页2019年7月29日 · We demonstrate that alpha-synuclein modulates DNA repair. First, alpha-synuclein colocalizes with DNA damage response components within discrete foci in human cells and mouse brain.
Fluorescence In Situ Hybridization (FISH) and Its Applications
网页The abbreviation ACM refers to the simultaneous hybridization of DNA probes for the alpha (centromere), classical (1q12) satellite and midi (1p36.3) satellite of chromosome 1 for the specific detection of duplications and deletions of 1pter and 1cen and for the identification of chromosomal breaks within the 1cen-1q12 region in human sperm.
CRISPR imaging reveals chromatin fluctuation at the centromere …
网页2023年9月5日 · Centromeric proteins were detected through immunostaining using anti-centromere antibody (CREST) staining to confirm whether GFP domains detected using CRISPR imaging targeting α-satellite DNA...
The ALFA-tag is a highly versatile tool for nanobody-based ... - Nature
网页2019年9月27日 · Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super ...
Alpha satellite DNA biology: finding function in the recesses of …
网页2018年7月5日 · This review will describe the genomic and epigenetic features of alpha satellite DNA, discuss recent findings of noncoding transcripts produced from distinct alpha satellite arrays, and address current progress in the functional understanding of this oft-neglected repetitive sequence.
Phosphorylation of the aggregate-forming protein alpha …
网页2021年11月23日 · Here we demonstrate using a combination of electrophoretic mobility shift assay and atomic force microscopy approaches that both aSyn and pSyn can bind DNA within the major groove, in a DNA length-dependent manner and with little specificity for …
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