Time-resolved fluorescence energy transfer - Wikipedia
网页Time-resolved fluorescence energy transfer (TR-FRET) is the practical combination of time-resolved fluorometry (TRF) with Förster resonance energy transfer (FRET) that offers a powerful tool for drug discovery researchers. TR-FRET combines the low background aspect of TRF with the homogeneous assay format of FRET.
HTRF——时间分辨荧光共振能量转移技术（TR-FRET）金标准 - 知乎
网页2024年2月29日 · HTRF——时间分辨荧光共振能量转移技术（TR-FRET）金标准. 什么是FRET？. 1946 年德国物理化学家Theodor Förster 首次提出荧光共振能量转移理论（FRET）并进行研究。. 直到 20 世纪 70 年代，FRET技术才被应用于生命科学和诊断领域。. 荧光共振能量转移（FRET）是指当 ...
Time-Resolved Fluorescence TRF / TR-FRET (HTRF) - Molecular …
网页Time-Resolved Fluorescence (TRF) and TR-FRET (HTRF) are the powerful tools for drug discovery which enable the analysis of molecular interactions in biochemical processes like kinase assays, cellular signaling pathways, protein-protein interactions.
TR-FRET技术应用指南 - univ-bio.com
网页TR-FRET（时间分辨荧光共振能量转移）技术，该技术结合了荧光共振能量转移（FRET，Fluorescence Resonance Energy Transfer）和时间分辨荧光（TRF，Time-Resolved Fluorescence）两种技术。HTRF®是首屈一指的TR-FRET。
荧光共振能量转移（FRET） - 知乎专栏
网页2023年5月23日 · FRET就是采用非放射方法，在供体和受体相互靠得很近（1-10 nm）时，将光子能从一个受激发的荧光团（供体）转移到另一个荧光团（受体）。 采用FRET，可以解决超过光学显微镜分辨率限制的分子相对邻近度问题，来显示（例如） （1）二个蛋白质成分间分子相互作用； （2）一个分子内部（如酶活动能力、DNA/RNA形态）的结构变化； （3）采用象CFP-YFP Cameleon这样特别的FRET-工具的离子浓度. CFP受光激发后便 …
Time-resolved Fluorescence Energy Transfer - Agilent
网页Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore.
Using TR-FRET to investigate protein-protein interactions: a case …
网页Time-resolved fluorescence resonance energy transfer (TR-FRET) protein-protein interaction assays, especially in the format of receptor-coregulator (coactivator and corepressor) recruitment/repression assays, have been widely used in nuclear receptor research to characterize the modes of action, efficacies, …
Development of a high-throughput TR-FRET screening assay for a …
网页2023年1月1日 · TR-FRET is a well-established platform for high-throughput screening (HTS), hit validation, and structure-activity relationship (SAR) studies [21]. We utilized this technology to develop an HTS-compatible TR-FRET assay to screen for small-molecule compounds that may potentially interfere with GDP/GTP exchange of …
时间分辨荧光能量转移 | 安捷伦 - Agilent
网页TR-FRET 可用于： BioTek Synergy Neo2 Hybrid 多功能微孔板检测仪. 具有独特 Hybrid 技术的 Hybrid 多功能微孔板检测仪. Category: 微孔板检测仪. BioTek Cytation 5 细胞成像多功能微孔板检测系统. 全自动宽场显微成像系统，以及基于光栅和滤光片的微孔板读数功能. Category: 细胞成像和显微检测系统. BioTek Cytation 1 细胞成像多功能微孔板检测系统. 可进行全自动宽场显微成像的细胞成像微孔板检测系统；可以进行基于滤光片的荧光、发光 …
Time-Resolved Fluorescence Resonance Energy Transfer [TR-FRET…
网页Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a fluorescence based technique which enables the analysis of molecular interactions in biochemical processes. Principle of TR-FRET is based on time-resolved fluorescence (TRF) measurement and fluorescence resonance energy transfer (FR ….
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