网页Expression of proliferation and survival signals by tumor cells allows the cells to grow continually as immortalized cells. To achieve growth independent of external growth factors, some tumor cells express activating mutations in proteins involved in cell
Circulating Tumor Cell Isolation, Culture, and Downstream …
网页Circulating tumor cells (CTCs) are a major contributor of cancer metastases and hold a promising prognostic significance in cancer detection. Performing functional and molecular characterization of CTCs provides in-depth knowledge about this lethal disease.
3D Cell Culture Systems: Tumor Application, Advantages, and ...
网页2021年11月11日 · Then we detail different types of natural supports such as matrigel, hydrogels, hard supports, and different synthetic strategies of 3D matrices such as lyophilization, electrospiding, stereolithography, microfluid by citing the advantages and disadvantages of each of them.
Three-dimensional cell culture: A powerful tool in tumor …
网页2017年10月3日 · In previous years, three-dimensional (3D) cell culture technology has become a focus of research in tumor cell biology, using a variety of methods and materials to mimic the in vivo microenvironment of cultured tumor cells ex vivo. These 3D tumor cells have demonstrated numerous different characteristics compared with traditional two ...
Cancer Cell Culture: Methods and Protocols | SpringerLink
网页There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters.
Cancer Cell Culture: The Basics and Two-Dimensional Cultures
网页From basic monolayer cultures and functional assays to more recent and ever-advancing cell-based cancer interventions, 2D cell culture plays a crucial role in cancer diagnosis, prognosis, and treatment.
Cancer Cell Culture: The Basics and Two-Dimensional Cultures
网页2023年5月19日 · Melissa Anne Tutty, Sarah Holmes & Adriele Prina-Mello,,, Part of the book series: Methods in Molecular Biology ( (MIMB,volume 2645)) 1032 Accesses. 4 Citations. Abstract. Despite significant advances in investigative and therapeutic methodologies for cancer, 2D cell culture remains an essential and evolving competency in …
Circulating tumor cells: biology and clinical significance
网页2021年11月22日 · Circulating tumor cells (CTCs) are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood. Understanding of the metastatic cascade of CTCs...
Organotypic culture assays for murine and human primary and …
网页2020年7月20日 · Organotypic 3D culture of fresh tumor tissue enables convenient real-time imaging, genetic and microenvironmental manipulation and molecular analysis. Here, we provide detailed...
Cancer Cell Culture: Methods and Protocols | SpringerLink
网页Adriele Prina-Mello. Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. Part of the book series: Methods in Molecular Biology (MIMB, volume 2645) 17k Accesses. 20 Citations. 8 Altmetric. About this book.
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