L-WRN conditioned medium for gastrointestinal epithelial stem …
网页We observed highly replicable activity levels among L-WRN CM batches prepared according to a previously published protocol. Quality control assays measuring spheroid growth or mRNA gene marker expression were best able to distinguish the quality L-WRN CM batches, whereas a Wnt reporter assay did not.
L-WRN conditioned medium for gastrointestinal epithelial stem …
网页The Pierce™ LDH Cytotoxicity Assay Kit (Thermofisher Scientific; Cat.# 88953) was used to assess cellular cytotoxicity according to manufacturer’s directions using L-WRN CM alone or the medium supernatant from spheroid cultures.
In vitro expansion and genetic modification of ... - Nature
网页2013年11月14日 · Gastrointestinal stem cells can be propagated by using conditioned medium from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned medium and how to culture...
In vitro expansion and genetic modification of gastrointestinal …
网页Gastrointestinal stem cells can be propagated by using conditioned medium from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned medium and how to culture stem cell-enriched epithelial spheroids from the mouse gastrointestine. These spheroids are also amenable to genetic modification with recombinant lentiviruses.
Culture and differentiation of rabbit intestinal organoids and …
网页2021年3月8日 · Our rabbit intestinal spheroid and monolayer cultures grew most efficiently in L-WRN-conditioned medium that contained Wnt, R-spondin and Noggin, and that had been supplemented with ROCK and...
Development of an enhanced human gastrointestinal epithelial …
网页The L-WRN CM supported the robust growth of mouse intestinal epithelial spheroids that were highly enriched for stem cells. Here, we overcame challenges associated with developing human intestinal spheroid culture protocols for patient-based assays by adapting our mouse method to the culture of human gastrointestinal epithelial cells.
L-WRN conditioned medium for gastrointestinal epithelial …
网页2019年5月1日 · Quality control assays measuring spheroid growth or mRNA gene marker expression were best able to distinguish the quality L-WRN CM batches, whereas a Wnt reporter assay did not. Thus, we have...
网页The L-WRN CM is a specialized biological reagent used to culture epi-thelial cells from multiple endodermal tissues of a variety of mammals, including mouse and human gastrointestinal epithelial stem cells. (Miyoshi and Stappenbeck, 2013; VanDussen et al., 2015; Aly et al., 2013; Powell and Behnke, 2017).
网页2013年11月14日 · Gastrointestinal stem cells can be propagated by using conditioned medium from a supportive cell line (l-Wrn). this protocol describes how to prepare conditioned medium and how to culture...
Live imaging analysis of human gastric epithelial spheroids reveals ...
网页Live imaging analysis revealed that spheroid growth was associated with cyclic rupture of the epithelial shell at a frequency of 0.32 ± 0.1/day, which led to the release of luminal contents. Spheroid rupture usually resulted in an initial collapse, followed by spontaneous re-formation of the spheres.