After fixation, the joints were dehydrated and subsequently embedded in paraffin. The tissue sections were stained with hematoxylin-eosin (H&E), and Safranin O-Fast Green (Saf-O/FG). 2.6.3 Fecal ...
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Because the endospore coat is so tough, steam is used to enable dye penetration. After washing, only the endospores will retain the primary stain Malachite green. Safranin is then used as a ...
Osteoarthritis is the most common joint disease in the world with significant societal consequences but lacks effective disease-modifying interventions. The pathophysiology consists of a prominent ...
Methods Progression of OA was analysed in both spontaneous and surgically induced OA models in wild type and PGRN-deficient mice. Cartilage degradation and OA were evaluated using Safranin O staining, ...
Coronal sections with a thickness of 4 μm were obtained through the knee joints. These sections were stained with Safranin O/Fast Green (G1371, Solarbio, Nanjing, China) according to the recommended ...
Methods Knee joints from an inbred C57/BL1/6 (ICRFa) mouse colony were harvested at 3–30 months of age. Sections were stained with H&E, Safranin-O, Picro-sirius red and antibodies to matrix ...