Fine-mapping the immunodominant antibody epitopes on consensus sequence ...
网页2022年11月25日 · Abstract. The HIV-1 envelope glycoprotein (Env) trimer is the key target for vaccines aimed at inducing neutralizing antibodies (NAbs) against HIV-1. The clinical candidate immunogen ConM...
HIV sequence database main page - Los Alamos National …
网页2023年3月20日 · The HIV Sequence Database contains all published and many unpublished HIV and SIV sequences. The sequence entries are richly annotated and fully searchable through several search interfaces. Sequence analysis tools are available for analyses of search results and user sequences.
Contemporary HIV-1 consensus Env with AI-assisted redesigned …
网页2024年5月9日 · HIV-1 Env consensus sequences that reflect recent sequences for clades B, C, and CRF01_AE were redesigned using AI-assisted methods to shorten hypervariable loops and limit strain-specific...
Updated HIV-1 Consensus Sequences Change but Stay Within …
网页2022年1月31日 · Here, we reconstruct 90 new HIV-1 subtype and CRF consensus sequences from 3,470 high-quality, representative, full genome sequences in the LANL HIV database. While subtypes and CRFs are unevenly spread across the world, in total 89 countries were represented.
HIV-1 Gag, Pol, and Env diversified with limited adaptation ... - mBio
网页2024年2月8日 · Our comprehensive sequence analysis of the diversity of structural proteins for HIV-1 subtypes and CRFs included 12,239 Env, Gag, and Pol sequences spanning 1980 to 2020. We found that, between 1980 and 2020, consensus sequences changed by an average of 5.3% AA in Env, 2.1% in Gag, and 2.9% in Pol, while sampled sequences became more distant ...
Predicted resistance to broadly neutralizing antibodies (bnAbs) …
网页2023年10月24日 · HIV-1 env sequences were used to predict bnAb resistance using bNAb-ReP, to determine the number of potential N-linked glycosylation sites (PNGS) and evaluate Env variable region...
Contemporary HIV-1 consensus Env with redesigned ... - bioRxiv
网页2023年11月19日 · Since Env with the longest hypervariable (HV) loops were more resistant to the cognate bnAbs than Env with shorter HV loops, we redesigned hypervariable loops for updated HIV-1 Env consensus sequences of subtypes B and C and circulating recombinant form AE (CRF01_AE).
Rapid Sequencing of Complete env Genes from Primary HIV-1 …
网页2016年7月8日 · Here, we demonstrate how this approach can provide an unprecedented view of high-quality, full-length HIV-1 env sequence populations derived from clinical samples. We used three different sets of samples: 1) the HIV infectious clone, NL4-3, to validate sequence quality, 2) a cross-sectional cohort …
Characteristics of HIV-1 env genes from Chinese chronically …
网页2020年12月1日 · We obtained env sequences in these samples through single genome amplification (SGA) assay. By comparing with hBCN-, subtype B chronically infected group (B-SP), and Chinese subtype B group (B-Database), we analyzed the characteristics of the env sequences of hBCN+ group.
Next-generation sequencing of HIV-1 single genome amplicons
网页2019年3月11日 · HIV-1 env has been a major focus of SGA amplicon sequencing efforts, due to the importance of the encoded glycoprotein (Gp160) in viral tropism and antibody-mediated immune responses [5,13]. Among HIV-1 structural genes, env presents the highest level of genetic diversity, with nucleotide sequences differing by up to 20% [http://www.hiv.lanl.gov/].