Guanidination of Tryptic Peptides without Desalting for Matrix …
网页2013年8月21日 · Guanidination, which converts lysine to homoarginine, an arginine analogue, can increase detection of those peptides 5–15-fold. Our aim was to improve guanidination by using a novel reagent, O-methylisourea-freebase. In a simple reaction, interfering salts were removed prior to guanidination.
[70] Guanidination of proteins - ScienceDirect
网页Guanidination is used to study the environment and essential nature of lysine side chains in proteins. The usefulness of guanidination can be demonstrated by the results of a few specific proteins such as guanidinated human serum albumin with O -methylisourea.
O-Methylisourea Can React with the α-Amino Group of Lysine ...
网页Guanidination of the ε-amino group of lysine-terminated tryptic peptides can be accomplished selectively in one step with O-methylisourea hydrogen sulfate. This reaction converts lysine residues into more basic homoarginine residues.
Integrating Lys-N proteolysis and N-terminal guanidination for …
网页Guanidination converts lysine residues into the more basic homoarginine [10, 12], which was shown to increase the sensitivity of detection of lysine-containing peptides following digestion with trypsin. Recently, Lys-N digestion was coupled with guanidination to improve peptide fragmentation behavior for doubly-charged ions in ETD.
Guanidination of Tryptic Peptides without Desalting for MALDI-TOF MS ...
网页2013年9月9日 · Guanidination, which converts lysine to homoarginine, an arginine analog, can increase detection of those peptides 5 – 15-fold. Our aim was to improve guanidination by using a novel reagent, O -methylisourea-freebase. In a simple reaction, interfering salts were removed prior to guanidination.
Guanidination chemistry for qualitative and quantitative …
网页2006年10月3日 · The application of guanidination chemistry, the conversion of lysine into homoarginine residues, is used to illustrate several important general considerations relating to the use of differential isotope labelling for relative quantification in proteomics.
Comprehensive Analysis of Protein N-Terminome by Guanidination …
网页2020年1月7日 · Herein, to solve this problem, we developed an approach, named terminal amine guanidination of substrates (TAGS). 1H-Pyrazole-1-carboxamidine was used to effectively guanidinate lysine ε-amines and N-terminal α-amines, followed by tryptic digestion to generate N-terminal peptides without free amines and internal peptides with free amines ...
An optimized guanidination method for large‐scale proteomic …
网页2016年5月13日 · As an ε–amine specific derivatization method, guanidination is widely used in proteomic studies for mainly two reasons: the significant improvement in ionization efficiency and the selective protection of ε–amine. Herein, we employed a systematic comparison of two widely used guanidination approaches and revealed the …
Guanidination of lysine residue improves the sensitivity and ...
网页2015年11月15日 · The guanidination reaction is specific to the ɛ-amine group of lysine and only occurs to a minimal extent at the N-terminal amine group [52]. More importantly, guanidination can transform a lysine side chain into a homoarginine side chain and can increase the basicity of the lysine side chain.
网页In this study, guanidination of previ-ously analyzed tryptic peptides was demonstrated, following efficient micro-recovery from the MALDI target. As one example, a tryptic digest of myoglobin was analyzed by MALDI-TOF MS (depicted in Figure 1). First, unguanidi-nated peptides were spotted and analyzed (Figure 1A).
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