Development of ISB 1442, a CD38 and CD47 bispecific ... - Nature
WEBMar 6, 2024 · ISB 1442 showed higher binding to CD38 + cells as compared to daratumumab in both high (Daudi and Raji) and low (NCI-H929 and KMS-12-BM) expressing CD38 + cells (Supplementary Fig. 5 and ...
Daratumumab induces CD38 internalization and impairs myeloma …
WEBJul 23, 2018 · For measuring CD38/anti-CD38 internalization, MM cell lines (MM.1S, NCI-H929, L363 and U266) were treated with 0.5 µg of mouse anti-human CD38 antibody (clone HB7, Biolegend) or 0.5 µg of Dara and incubated for 1 hour at 37⁰C.
Epigenetic regulation of CD38/CD48 by KDM6A mediates NK cell …
WEBFeb 14, 2024 · We performed another genome-wide CRISPR KO screen to identify the genes regulating CD38 expression in H929 cells (Fig. 2a).
CD38-Induced Metabolic Dysfunction Primes Multiple Myeloma …
WEBFeb 15, 2023 · Metabolic reprogramming elicited by CD38-overexpression identifies a novel druggable vulnerability in MM cells. (A) Cellular and mitochondrial NAD + contents were measured in H929 CD38 OE or control cells and normalized to the protein content
Identification a Novel Molecular Mechanism Underlying the Anti-CD38 …
WEBNov 15, 2022 · To determine whether CD38 splice variants encode functional proteins, we cloned, sequenced, and stably expressed CD38 SpVs transcripts into H929 cells (H929-CD38 SpVs). CD38 SpVs expressions were evaluated by flow cytometry and live cell imaging. These analyses demonstrated that CD38 SpVs encode proteins.
CD38 deletion of human primary NK cells eliminates …
WEBNov 19, 2020 · We used the CRISPR/Cas9 system to delete CD38 (CD38 KO) in ex vivo expanded peripheral blood NK cells. These CD38 KO NK cells were completely resistant to DARA-induced fratricide, showed superior persistence in immune-deficient mice pretreated with DARA, and enhanced ADCC activity against CD38-expressing MM cell lines and primary MM cells.
Development of a reporter gene method to measure the bioactivity …
WEBOct 8, 2021 · The results demonstrate that the gene reporter intensity, which impacts the assay sensitivity, is a dependence of the CD38 levels on the target tumor cells. Therefore, NCI-H929 with the highest CD38 expression among the three tested tumor cells was chosen as the target cell for Y150 bioassay development.
BCMA-Specific ADC MEDI2228 and Daratumumab Induce …
WEBOct 1, 2021 · M2 significantly induces expression of IRF1 via STAT1 activation to sustain CD38 upregulation in multiple myeloma cells. As in H929 and RPMI8226 cells, M2 treatment also consistently induced STAT1 phosphorylation, CD38 expression, and increased γH2AX in MM1S and OPM2 cells (Fig. 3A; Supplementary Fig. S1B).
The JAK-STAT pathway regulates CD38 on myeloma cells in the …
WEBNov 12, 2020 · Key Points. Bone marrow stromal cells from myeloma patients downregulate CD38 expression on myeloma cells through activation of the JAK-STAT3 pathway. JAK inhibitor ruxolitinib upregulates CD38 expression on myeloma cells and enhances daratumumab-mediated cytotoxicity.
CD38 and Regulation of the Immune Response Cells in Cancer
WEBCD38 is a multifunctional ectoenzyme that functions as a nicotinamide adenine dinucleotide (NAD+) glycohydrolase and catalyzes the synthesis and degradation of cADPR affecting calcium signaling and release, thus decreasing extracellular NAD+, altering calcium cascade and deeply contributing to adenosine-mediated immune …